ifn β  (Proteintech)

Journal: Oncoimmunology

Article Title: Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model

doi: 10.1080/2162402X.2025.2602216

Figure Lengend Snippet: (A–I) Representative images of cGAS, STING, pSTAT1, IFNβ, CCL5, CXCL10, HMGB1, PD-L1, and γH2AX immunofluorescent or immunohistochemical stained tissue sections, respectively, of untreated, αPD-L1 mAb-treated, Ht-treated and Ht+αPD-L1 mAb-treated tumors (treatment side and abscopal side).

Article Snippet: The following antibodies were used for western blotting and immunostaining: anti-human cGAS (Cell Signaling), anti-mouse cGAS (SantaCruz), STING (Novus Biological), phospho-IRF3 Ser-386 (Cell Signaling), phospho-IRF3 Ser-396 (Cell Signaling), IRF-3 (SantaCruz), phospho-TBK1 Ser172 (Cell Signaling), TBK1 (SantaCruz), phospho-H2A.X Ser139 (Cell Signaling), PD-L1 (Cell Signaling), CD11c (Cell Signaling), CD8α (Cell Signaling), F4/80 (Proteintech), CD163 (Proteintech), CD86 (Novus Biologicals), FoxP3 (Cell Signaling), Gr1 (Invitrogen), GranzymeB (Invitrogen), Tim3 (Invitrogen), phospho-STAT1 Tyr701 (Cell Signaling), and IFNβ, CCL5, CXCL10, CD56, MICA, HMGB1, SUMO1, SUMO2/3, UBC9, SAE1, UBA2, SENP3, Beta-Actin (all from Proteintech).

Techniques: Immunohistochemical staining, Staining

Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: IL1RA is associated with oncogenic signaling pathways and type I interferon response in OSCC in vitro . A. Volcano plots visualizing 132 DEGs between CAL27 cells transfected with NC and oeIL1RA. Down-regulated, up-regulated and non-regulated genes were labeled in blue, red, and grey colors, respectively. B. GSEA showed less enriched cancer-related features or processes in CAL27 cells overexpressing IL1RA. C. GSEA showed that IL1RA overexpression was significantly associated with the type I interferon response. D. A Venn diagram visualizing 45 (61.6 %) OSCC patients simultaneously carrying mutations in the IL1RA, IFNA , and IFNB genes in the TCGA dataset.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Protein-Protein interactions, In Vitro, Transfection, Labeling, Over Expression

Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: Overexpression of IL1RA up-regulates type I interferon proteins in OSCC in vitro . A. H&E (the first lane) and IHC staining (the latter three lanes) of IFNA and IFNB in OSCC specimens of the high and low IL1RA expression groups (n=30, scale bar=100 μm). B-C. The mRNA (B) and protein expressions (C) of IFNA and IFNB in OSCC specimens of the high and low IL1RA expression groups. * P <0.05, and ** P <0.01 vs. NC group.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Over Expression, In Vitro, Immunohistochemistry, Expressing

Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: IL1RA promotes the expressions of IFNA and IFNB in the OSCC xenografts and their release in OSCC cells. A. IHC staining of positive expressions of IFNA and IFNB in OSCC xenografts of oeIL1RA group and NC group in vivo (scale bar=100 μm). B. The mRNA levels of IFNA and IFNB in OSCC xenografts of the oeIL1RA group and NC group in vivo . C. ELISA showed contents of IFNA and IFNB in the cell supernatant of the oeIL1RA group and NC group in vitro . * P <0.05, and ** P <0.01 vs. NC group.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Immunohistochemistry, In Vivo, Enzyme-linked Immunosorbent Assay, In Vitro